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KMID : 0378019710140070055
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New Medical Journal
1971 Volume.14 No. 7 p.55 ~ p.62
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Effect of Single or Franctionated In Vitro X-Irradiation on the NP-SH and NP-SS of Rabbit Liver and Lung Tissues
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Abstract
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In view of the importance of sulfhydryl groups in producing a chemical protection against ionizing radiation, an attempt was made to evaluate the changes of intrinsic non-protein sulfhydryl (NP-SH) and non-protein disulfide (NP-SS) of the lung and liver tissues of rabbits after subjecting the tissues either to single or fractionated (300r x 3) X-irradiation with 900r in vitro.
NP-SH was measured by Ellman´s method and NP-SS was measured by the electrolytic reduction method described by Dohan and Woodward.
The experiment was performed at 1, 3, 5, 24 and 48 hours post-irradiation, and the results were compared with the control.
The results thus obtained are summarized as follows:
1. Intrinsic levels of NP-SH and NP-SS of the normal rabbit were 7.70±0. 10 and 6.09±0.70 p mol/ gm wet wt. in the liver, and 2.79±0.30 and 5.40±0.30 p mol/gm wet wt. in the lung, respectively.
2. NP-SH of the liver after the irradiation showed a marked decrease throughout the experiment, and the pattern of decrease was more prominent in the fractionated group than in the single irradiated group.
3. NP-SH levels of the lung after the single irradiation showed elevated values comparing with the control (i.e., normal) value in general, but in the fractionated group, NP-SH showed no significant change from the normal. However, comparing with the single irradiated group, NP-SH of the fractionated group showed markedly decreased values.
4. Levels of NP-SS in the liver decreased significantly from the normal throughout the experiment, but there was no marked difference in NP-SS between the single and fractionated group especially after 5 hours post-irradiation.
5. NP-SS of the lung in both the single and fractionated groups showed significant decreases from the normal, and though the decrease was more prominent in the fractionated group, the difference was not significant.
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